ABSTRACT
Objective:To explore the apoptosis inducing effect of matrine on C6 glioma cells and the related mechanisms.Methods:MTT assay was used to examine the inhibition of C6 glioma cell line by matrine at various concentrations and the IC50 was calculated.Inverted microscope and TEM(transmission electron microscope) were employed to observe the morphological alterations of C6 glioma cells after exposure to matrine;FCM(Flow cytometry) was used to detect the apoptosis rate of C6 glioma cells;and real-time PCR was used to examine the differential expression of related genes.ICC and Western blotting was used to detect the expression of caspase-3.Results:MTT showed that the cell inhibition effect of matrine increased with its concentration(0.1-1.0 mg/ml)(P
ABSTRACT
Objective: To evaluate the pharmacodynamic of clematis. Methods: 1) The relaxation of isolated ileum muscle of guinea pig by clematic was tested. The antagonism of clematis on histamine or Ach induced ileum muscle contraction was tested also. 2) By using the model of turning of trunk, the pain releasing effect of clematis was evaluated. 3) The experiments about anti-inflammation action of clematis were carried out in two models. Results: Clematis relaxed the ileum muscle. It also antagonized the contraction of the muscle caused by histamine and Ach. A single intrapeditional administration of clematis to Kun-Ming mice significantly prolonged the latency and reduced the writhe number at the turning of trunk model; clematis dose-dependently inhibited the mouse ear swelling caused by xylol. It also had anti-inflammation effect at the other model. The results demonstrated that clematis could release the pain, suppress the inflammation and smooth muscle contraction.